Fig. 7

Effects of STEAP4 overexpression and 50 μM copper on cell cycle distribution in Hep G2 cells. A–C Cell cycle distribution analysis shows that co-treatment with STEAP4 overexpression and 50 μM copper increases the percentage of Hep G2 cells in sub-G1 and G2/M phases. The synergistic effect is eliminated by STEAP4 knockdown via siRNA, indicating G2/M cell cycle arrest associated with STEAP4 copper reductase activity. * p < 0.05