Fig. 5

miR-3154 promotes glioblastoma cells proliferation and metastasis via targeting TP53INP1. A. 3’UTR of TP53INP1 was a predicted potential binding site of miR-3154 identified by TargetScan. Except wildtype TP53INP1, a mutant TP53INP1 was also constructed to disrupt the interaction between miR-3154 and TP53INP1 mRNA. B. Dual luciferase reporter gene assays were performed on U251 miR-3154 sponge or A172 miR-3154 sponge and their control cells transfected with wild-type or mutant TP53INP1 3’-UTR. *p < 0.05. C. mRNA expression of TP53INP1 was measured by qRT-PCR in miR-3154 sponge U251 or miR-3154 sponge A172 and control cells. *p < 0.05. D. Protein expression of TP53INP1 was measured by qRT-PCR in miR-3154 sponge U251 or miR-3154 sponge A172 and control cells. E. Proliferation ability of miR-3154 sponge or control cells transfected with si-TP53INP1 or si-NC were measured in both U251 and A172 cell lines at indicated timepoints using CCK8 assay. *p < 0.05. F. Proliferation ability of miR-3154 sponge or control cells transfected with si-TP53INP1 or si-NC were measured in U251 cell line using colony formation assay. G. Proliferation ability of miR-3154 sponge or control cells transfected with si-TP53INP1 or si-NC were measured in A172 cell line using colony formation assay. H. Migration ability of miR-3154 sponge or control cells transfected with si-TP53INP1 or si-NC were measured in U251 cell line using invasion assay. I. Migration ability of miR-3154 sponge or control cells transfected with si-TP53INP1 or si-NC were measured in A172 cell line using invasion assay. J&K. The indicated cells (1 × 10⁷) were subcutaneously injected into nude mice (n = 6) for xenograft assay. Tumor growth curve and average weight in each group was shown. L. The expression of miR-3154 and TP53INP1 in xenografted tumors detected by qRT-PCR. *p < 0.05