Fig. 2

miR-3154 promotes glioblastoma cells proliferation. A. knockdown effect of miR-3154 sponge in U251 and A172 cell lines detected by qRT-PCR. *p < 0.05. B. Proliferation curve at indicated timepoint (24 h, 48 h, 72 h, 96 h) was measured by CCK8 assay. *p < 0.05. C. Photographs of colonies formed by miR-3154 sponge transfected cells and control cells were captured under microscope. D. Cell cycle analysis was performed on miR-3154 sponge transfected cells and control cells using flow cytometry. The percentage in histogram (left) are shown as bar chart (right). E. Protein expression of cyclin D1, cyclin E1 and PCNA were detected by western blot. F&G. U251 miR-3154 sponge or control cells (1 × 10⁷) were subcutaneously injected into nude mice (n = 6) for xenograft assay. Tumor growth curve and average weight in each group was shown. H. Knockdown effect of miR-3154 sponge in xenografted tumors detected by qRT-PCR. *p < 0.05