Fig. 8
LAMA4 promoted the biological function via COL4A1.A Western blot analysis of COL4A1 overexpression efficiency in Hep3B/DDP and Huh- 7/DDP cells. B IC50 values of Hep3B/DDP and Huh- 7/DDP cells after transfection with si-NC, si-LAMA4, si-LAMA4 + vector, si-LAMA4 + COL4A1. C, D The expression of MDR1 and MRP1 in Hep3B/DDP and Huh- 7/DDP cells with si-NC, si-LAMA4, si-LAMA4 + vector, si-LAMA4 + COL4A1 was analyzed by western blot. E MTT assay of cell viability in Hep3B/DDP and Huh- 7/DDP cells after transfection with si-NC, si-LAMA4, si-LAMA4 + vector, si-LAMA4 + COL4A1. F EdU assay of cell proliferation in Hep3B/DDP and Huh- 7/DDP cells after transfection with si-NC, si-LAMA4, si-LAMA4 + vector, si-LAMA4 + COL4A1. G, H Flow cytometry assay of apoptosis in Hep3B/DDP and Huh- 7/DDP cells after transfection with si-NC, si-LAMA4, si-LAMA4 + vector, si-LAMA4 + COL4A1. I Transwell assay was used for cell invasion in Hep3B/DDP and Huh- 7/DDP cells after transfection with si-NC, si-LAMA4, si-LAMA4 + vector, si-LAMA4 + COL4A1. J Cell migration of cells was detected by wound healing in Hep3B/DDP and Huh- 7/DDP cells after transfection with si-NC, si-LAMA4, si-LAMA4 + vector, si-LAMA4 + COL4A1. *P < 0.05