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Fig. 3 | Cell Division

Fig. 3

From: YAP1 activates SLC2A1 transcription and augments the malignant behavior of colorectal cancer cells by activating the Wnt/β-catenin signaling pathway

Fig. 3

YAP1 transcriptionally activates SLC2A1.A–B Predicted binding results of YAP1 to the SLC2A1 promoter from the JASPAR database and YAP1’s conserved binding sequence; C Co-transfection of the luciferase reporter vector containing the SLC2A1 promoter sequence and YAP1 overexpression vector in HEK293T cells, measuring luciferase activity; D ChIP assay using anti-YAP1, followed by qPCR analysis of SLC2A1 promoter levels in the precipitate; E, F qPCR and WB analyses of YAP1 levels in COAD cell lines Caco-2, HCT116, SW480, and the immortalized colonic mucosal cell line FHC; G Comparison of YAP1 levels in TCGA-COAD and normal colon tissues from the GTEx database; H Correlation between YAP1 and SLC2A1 levels in the TCGA-COAD database; I, J qPCR and WB analyses of YAP1 and SLC2A1 levels in SW480 and Caco-2 cells

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Cell Division

ISSN: 1747-1028

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