Fig. 6

YAP activator reverses the inhibitory effect of overexpression of RFX2 on LUAD cells. A WB analysis of YAP protein expression and its phosphorylation after addition of PY-60 in A-549 and NCI-H358 cells; B CCK-8 assay for cell viability; C Transwell assay for cell invasion; D TUNEL assay for cell apoptosis; E RT-qPCR analysis of PD-L1 mRNA expression; F ELISA to detect the levels of IFN-γ, GZMB, and PRF1. All cellular experiments were independently repeated 3 times. Comparisons between 2 groups of a single factor were performed by unpaired t-test (ACDE); comparisons among multiple groups of a single factor were performed by one-way ANOVA (F); comparisons between 2 groups of two factors were performed by two-way ANOVA (B)