Fig. 4

RFX2 regulates RASSF1 transcription in LUAD progression. A mRNA expression of RASSF1 detected by RT-qPCR; B Proliferative ability of A-549 and NCI-H358 cells assessed by CCK-8 assay; C Invasive ability of A-549 and NCI-H358 cells determined by Transwell assay; D Apoptosis of A-549 and NCI-H358 cells determined by TUNEL assay; E Tumor volume analysis to assess the effect of overexpression of RFX2 and knockdown of RASSF1 on tumor growth; F Weight of tumors formed by LA-795 mouse LUAD cells; G RFX2 and RASSF1 expression in mouse transplanted tumor tissues detected by IHC; H CD8 and Ki-67 expression in transplanted tumor tissues detected by immunohistochemistry. All cellular experiments were independently repeated 3 times, and all animal experiments had 5 mice in each group. Unpaired t-test (ACD) was used for the comparison between 2 groups of a single factor, one-way ANOVA (FGH) for the comparison among multiple groups of a single factor, and two-way ANOVA (BE) for the comparison between 2 groups of two factors