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Fig. 3 | Cell Division

Fig. 3

From: RFX2 downregulates RASSF1 expression and YAP phosphorylation through Hippo signaling to promote immune escape in lung adenocarcinoma

Fig. 3

Low RFX2 expression downregulates the expression of the downstream target RASSF1. A Intersection of DEGs of GSE32863, GSE43458, GSE21933 datasets, top 50 targets of RFX2 and the first 2500 DEGs of LUAD in GEPIA obtained by Jvenn; B TIMER2.0 analysis of the correlation between RASSF1 and the infiltration of CD8+ T cells; C Proteomics analysis of RFX2 in LUAD by proteomics in UALCAN; D hTFtarget prediction of RFX2 downstream targets; E GEPIA correlation analysis of RFX2 with RASSF1; F IHC experiments to analyze RASSF1 expression in clinical LUAD tissue samples; G Correlation between RFX2 and RASSF1 protein expression; H Dual-luciferase assay confirmed the correlation between interaction between RFX2 and the RASSF1 promoter in A-549 and NCI-H358 cells; I RT-qPCR to detect mRNA expression of RASSF1 in the oe-NC and oe-RFX2 groups; J ChIP assay verified that RFX2 could bind to RASSF1 promoter in A-549 and NCI-H358 cells. All cell experiments were independently repeated 3 times. Single-factor comparison between the 2 groups was performed by t-test (FHIJ)

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Cell Division

ISSN: 1747-1028

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