Fig. 2

RFX2 overexpression induces CD8⁺ T cell activation and inhibits LUAD cell immune escape and malignant phenotype. A RFX2 expression correlates with the infiltration of CD8⁺ T cells in LUAD and LUSC, respectively; B WB analysis of RFX2 protein expression in normal lung cells and LUAD cell lines; C RT-qPCR to detect oe-RFX2 efficiency in A-549 and NCI-H358 cells; D WB analysis of RFX2 protein expression in A-549 and NCI-H358 cells in the oe-NC and oe-RFX2 groups; E ELISA for IFN-γ, GZMB, and PRF1 contents in the oe-NC and oe-RFX2 groups; F WB analysis of PD-L1 expression in cells of the oe-NC and oe-RFX2 groups; G CCK8 assay for A-549 and NCI-H358 cell proliferation ability; H Transwell assay for invasion of A-549 and NCI-H358 cells overexpressing RFX2; I TUNEL assay for apoptosis of A-549 and NCI-H358 cells overexpressing RFX2. All cellular experiments were independently repeated 3 times. Comparisons between 2 groups of a single factor were performed by unpaired t-test (C); comparisons among multiple groups of a single factor were performed by one-way ANOVA (BDEFHI); comparisons among multiple groups of two factors were performed by two-way ANOVA (G)