Fig. 5
From: LncRNA-ANRIL regulates CDKN2A to promote malignant proliferation of Kasumi-1 cells
CDKN2A downregulation suppressed cell proliferation and disrupted cell cycle progression via the GSK3β/β-catenin/cyclin D1 signaling pathway. A. RT-qPCR analysis confirmed the knockdown efficiency of CDKN2A. B. CCK-8 assay evaluated cell proliferation rates post-knockdown. C, D. Flow cytometry revealed cell cycle alterations following CDKN2A silencing. E, F. Western blotting analysis demonstrated the expression levels of CDK2, p-CDK2, CDK4, CDK6, cyclin D1, GSK3β, and β-catenin upon CDKN2A downregulation. G. RT-qPCR validation of CDKN2A knockdown efficiency post co-transfection with sh-CDKN2A and pcDNA-ANRIL. H, I. Western blotting assessed CDK2, p-CDK2, CDK4, CDK6, cyclin D1, GSK3β, and β-catenin protein expression following co-transfection of sh-CDKN2A and pcDNA-ANRIL. ns P>0.05, *P < 0.05, **P < 0.01, ***P < 0.001