Fig. 3
From: LncRNA-ANRIL regulates CDKN2A to promote malignant proliferation of Kasumi-1 cells
Impact of ANRIL upregulation reversal by Palbociclib on Kasumi-1 cells. A. CCK-8 assay determines the optimal Palbociclib concentration by treating Kasumi-1 cells with 0, 1, 5, 10, 15, 20, and 25 µmol for 24 h. B, C. Flow cytometry reveals cell cycle distribution following treatment with 15 µmol Palbociclib for 24 h. D, E. Flow cytometry reveals cell cycle distribution following treatment with 15 µmol Palbociclib for 48 h. F, G. Western blotting analysis assesses CDK2, p-CDK2, CDK4, CDK6, and cyclin D1 protein expression after palbociclib treatment. ns P>0.05, *P < 0.05, **P < 0.01, ***P < 0.001