Fig. 2
From: LncRNA-ANRIL regulates CDKN2A to promote malignant proliferation of Kasumi-1 cells
The ANRIL-regulated GSK3β/β-catenin/cyclin D1 signaling pathway modulated Kasumi-1 cell cycle progression. A, B. Validation of ANRIL downregulation and upregulation efficiency. C, D, E, F. Flow cytometry analysis demonstrating cell cycle distribution under ANRIL upregulation and downregulation. G, H. RT-qPCR analysis quantifying mRNA levels of CDK2, CDK4, CDK6, cyclin D1, GSK3β, and β-catenin following ANRIL modulation. I, J, K, L. Western blotting evaluation of ANRIL expression effects on protein levels of CDK2, p-CDK2, CDK4, CDK6, cyclin D1, GSK3β, and β-catenin. M, N. Flow cytometry analysis demonstrating cell cycle distribution following the co-transfection of ANRIL overexpression and cyclin D1 suppression. *P < 0.05, **P < 0.01, ***P < 0.001