Fig. 6

Combined knockdown of STIL and FOXM1 regulates SF3A3 transcription to suppress HCC cell growth in vivo. (A) Measurement of tumor volume changes in mice injected with KD-NC-, KD-SF3A3-, and KD-STIL-treated Huh7 cells. (B) Measurement of tumor volume changes in mice injected with KD-STIL + KD-NC-, KD-STIL + KD-FOXM1-, KD-STIL + OE-NC-, and KD-STIL + OE-SF3A3-treated Huh7 cells. (C) Changes in tumor weight in the KD-NC, KD-SF3A3, and KD-STIL groups. (D) Changes in tumor weight in the KD-STIL + KD-NC, KD-STIL + KD-FOXM1, KD-STIL + OE-NC, and KD-STIL + OE-SF3A3 groups. (E) The mRNA expression of SF3A3 in the tumor tissues of mice in the seven groups. (F) Expression of Ki67, GPC3, and p53 in the KD-NC, KD-SF3A3, and KD-STIL groups was detected by immunohistochemistry. (G) Expression of Ki67, GPC3, and p53 in the KD-STIL + KD-NC, KD-STIL + KD-FOXM1, KD-STIL + OE-NC, and KD-STIL + OE-SF3A3 groups was detected by immunohistochemistry. All data are expressed as mean ± SD (n = 5). In panels A-B, significance is determined by two-way ANOVA; in panels C-G. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001