Fig. 4

An interaction between STIL and FOXM1 induces SF3A3 expression. (A) The presence of binding sites for FOXM1 at the promoter of SF3A3 was predicted in the hTFtarget database. The positive correlation between FOXM1 (B) and STIL (C) with SF3A3 expression in the GEPIA database. (D) The binding relationship between STIL and FOXM1 was analyzed using co-IP. (E) FOXM1 mRNA expression in HCC cell lines Hep3B and Huh7 treated with KD-FOXM1 or KD-NC using RT-qPCR. (F) The effect of KD-STIL alone or in combination with KD-FOXM1 on FOXM1 binding at the SF3A3 promoter was analyzed using ChIP. (G) Changes in SF3A3 promoter luciferase activity were analyzed using dual-luciferase reporter assays. (H) SF3A3 mRNA expression after downregulation of STIL alone or combined downregulation of FOXM1 and STIL detected by RT-qPCR. (I) FOXM1 protein expression in Hep3B and Huh7 cells after knockdown of STIL. All data are expressed as mean ± SD from three experiments. In panels E-I, significance is determined by two-way ANOVA. **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant