Fig. 3

STIL is overexpressed in HCC and knockdown of STIL inhibits the malignant behavior of HCC cells. (A) The intersection of SF3A3-positively correlated genes and prognostic markers in the UALCAN database, and differentially expressed genes in the GSE202853 dataset. (B) Transcription level of STIL (transcript per million) in primary tumor tissues and normal tissues of HCC patients in the UALCAN database. (C) The survival of HCC patients with high STIL expression (n = 90) or medium/low STIL expression (n = 275). (D) STIL mRNA expression in tumor tissues and corresponding adjacent tissues from HCC patients using RT-qPCR (n = 30). (E) STIL mRNA expression in HCC cell lines Hep3B and Huh7 and normal hepatocyte cell line THLE-2 using RT-qPCR. (F) STIL mRNA expression in HCC cell lines Hep3B and Huh7 treated with KD-STIL or KD-NC using RT-qPCR. (G) SF3A3 mRNA expression in HCC cell lines Hep3B and Huh7 treated with KD-STIL or KD-NC using RT-qPCR. (H) STIL mRNA expression in HCC cell lines Hep3B and Huh7 treated with KD-SF3A3 or KD-NC using RT-qPCR. Evaluation of cell proliferation using EdU assay (I) and colony formation assay (J) in Hep3B and Huh7 cells. (K) Detection of apoptosis using flow cytometry in Hep3B and Huh7 cells. Analysis of cell migration and invasion by wound healing assay (L) and Transwell invasion assay (M) in Hep3B and Huh7 cells. All data are expressed as mean ± SD from three experiments. In panel D, significance is determined by paired t-test; in panel E, significance is determined by one-way ANOVA; in panel F-M, significance is determined by two-way ANOVA. **p < 0.01, ***p < 0.001, ****p < 0.0001