Fig. 6

Increased cell proliferation mediated by ZNF169 overexpression is attenuated by FBXW10 knockdown. (A) Western blot analysis of TPC1 cells transfected with shCtrl + Ctrl, shZNF169 and shZNF169 + FBXW10 overexpression plasmid. GAPDH was used as the loading control. (B) Cell Counting Kit-8 analysis of TPC1 cells transfected with shCtrl + Ctrl, shZNF169 and shZNF169 + FBXW10 overexpression plasmid. (C) Colony formation assay of TPC1 cells transfected with shCtrl + Ctrl, shZNF169 and shZNF169 + FBXW10 overexpression plasmid. (D) Western blot analysis of K1 cells transfected with control, ZNF169 overexpression plasmid and ZNF169 overexpression plasmid + shFBXW10#1. GAPDH was used as the loading control. (E) Cell Counting Kit-8 analysis of K1 cells transfected with control, ZNF169 overexpression plasmid and ZNF169 overexpression plasmid + shFBXW10#1. (F) Colony formation assay of K1 cells transfected with control, ZNF169 overexpression plasmid and ZNF169 overexpression plasmid + shFBXW10#1. (G) Representative images of subcutaneous xenografts in nude mice derived from K1 cells that were treated with control, ZNF169 overexpression plasmid and ZNF169 overexpression plasmid + shFBXW10#1. n = 5 mice per group. The subcutaneous xenografts were dissected and are shown on day 25. (H) Growth curves of the subcutaneous xenografts in each group. (I) Analysis of the tumor weight of the xenografts in each group. ^**P < 0.01, ^***P < 0.001