Fig. 3

ZNF169 overexpression promotes cell proliferation and migration. (A, B) Overexpression of ZNF169 in K1 cells. RT-qPCR and western blot analyses demonstrated the efficiency of transfection with the ZNF169 overexpression plasmid and Ctrl in K1 cells. β-actin was used for normalization and GAPDH was used as the loading control. (C) Cell Counting Kit-8 analysis of K1 cells transfected with the ZNF169 overexpression plasmid and Ctrl. (D) Colony formation assay of K1 cells transfected with the ZNF169 overexpression plasmid and Ctrl. (E) Transwell invasion analysis of K1 cells transfected with the ZNF169 overexpression plasmid and Ctrl. Magnification: 100 x. ^**P < 0.01, ^***P < 0.001