Fig. 2

ZNF169 knockdown suppresses cell proliferation and migration. (A) RT-qPCR demonstrated the efficiency of transfection with shCtrl, shZNF169#1 and shZNF169#2 in BCPAP and TPC1 cells. β-actin was used for normalization. (B) Western blot analyses demonstrated the efficiency of transfection with shCtrl, shZNF169#1 and shZNF169#2 in BCPAP and TPC1 cells. GAPDH was used as the loading control. (C) Cell Counting Kit-8 analysis of BCPAP and TPC1 cells transfected with shCtrl, shZNF169#1 and shZNF169#2. (D) Colony formation assay of BCPAP and TPC1 cells transfected with shCtrl, shZNF169#1 and shZNF169#2. (E) Transwell invasion analysis of BCPAP and TPC1 cells transfected with shCtrl, shZNF169#1 and shZNF169#2. ^**P < 0.01, ^***P < 0.001