Fig. 3

Curcumin and ATG3 Knockdown Modulate Autophagy in Cervical Cancer Cells (A) Immunofluorescence staining of LC3 in SiHa cells under control, ShATG3, curcumin, and ShATG3 combined with curcumin treatments. DAPI stains nuclei (blue), and LC3 is shown in green. Merged images indicate the co-localization of LC3 with DAPI. (B) Quantification of LC3 fluorescence intensity and protein levels in SiHa cells. The relative fluorescence intensity of LC3 significantly increased in curcumin and ShATG3 + curcumin groups compared to control and ShATG3 groups. Western blot analysis showing LC3-I and LC3-II levels in SiHa cells under different treatments. (C) Immunofluorescence staining of LC3 in HeLa cells under control, ShATG3, curcumin, and ShATG3 combined with curcumin treatments. DAPI stains nuclei (blue), and LC3 is shown in green. Merged images indicate the co-localization of LC3 with DAPI. (D) Quantification of LC3 fluorescence intensity and protein levels in HeLa cells. The relative fluorescence intensity of LC3 significantly increased in curcumin and ShATG3 + curcumin groups compared to control and ShATG3 groups. Western blot analysis showing LC3-I and LC3-II levels in HeLa cells under different treatments. Statistical significance is indicated by **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: not significant