Fig. 6
From: ZEB2 reduction contributes to pre-eclampsia via Wnt/β-Catenin pathway
Influence of AV939 and ZEB2-plasmid on trophoblast cells proliferation, apoptosis, migration and invasion EdU assay was applied for determining trophoblast cells growth. (E) Plot showing the quantification of EdU+ cells. (C) Flow cytometry analysis of apoptotic cells. (D) Quantitation of apoptotic cells. (E) Western blot analysis of Bax and Bcl-2 expression. (F) the quantification of Bax and Bcl-2 protein expression. (G) Relative mRNA levels of Bax and Bcl-2 were assessed by RT-qPCR analysis. H and I) Cells migration was detected using transwell assay. (J and K) Invasion capacity of HTR-8/SVneo cells was assessed by transwell assay. * P < 0.05, **P < 0.01