Fig. 2
From: ZEB2 reduction contributes to pre-eclampsia via Wnt/β-Catenin pathway
Effects of ZEB2-plasmid on HTR-8/SVneo cells proliferation and apoptosis. Trophoblast cells were transfected with control-plasmid or ZEB2-plasmid, and cells were divided into three groups: Control, control-plasmid and ZEB2-plasmid. (A) Relative ZEB2 levels were determined by RT-qPCR analysis. (B) Western blot analysis of ZEB2 expression. (C) Plot showing the quantification of ZEB2 expression. (D) The proliferation of trophoblast cells were determined using EdU assay. (E) Plot showing the quantification of EdU+ cells. (F) Flow cytometry analysis of apoptotic cells. (G) Quantitation of apoptotic cells. (H) Western blot analysis of Bax and Bcl-2 expression. (I) the quantification of Bax and Bcl-2 protein expression. (J) Relative mRNA levels of Bax and Bcl-2 were assessed by RT-qPCR analysis. **P < 0.01