Fig. 5

Ectopic expression of YAP1 reverses the functional phenotypes induced by RNF6 deficiency in CC. (A) Western blotting analysis detected the protein expression of RNF6 and YAP1 in RNF6-silenced HeLa cells co-transfected with or without the YAP1 overexpression plasmid. (B-D) CCK-8 assays and colony formation assays were conducted using RNF6-silenced HeLa cells co-transfected with or without the YAP1 overexpression plasmid. (E-G) Transwell invasion and wound healing assays were utilized to assess the migratory and invasive capacities of RNF6-silenced HeLa cells co-transfected with or without the YAP1 overexpression plasmid. *p < 0.05, **p < 0.01