Fig. 4

RNF6 exerts regulatory effects on the YAP signaling pathway. Transfection of the RNF6 overexpression plasmid was conducted in HeLa cells, followed by transcriptome sequencing. (A) A heatmap illustrating the overall expression pattern of select differentially expressed genes. (B) Expression profile of all genes, with 137 genes upregulated (in red) and 131 genes downregulated (in blue) depicted in a volcano plot. (C) KEGG enrichment analysis for differentially expressed genes presented in bubble plots. (D, E) The protein and mRNA expression of YAP1, and its downstream target genes CYR61 and CTGF detected by Western blot and qRT‒PCR in CC cells following modulation of RNF6 expression. *p < 0.05, **p < 0.01