Fig. 2

Elevated levels of RNF6 triggers the aggressive characteristics of CC cells. (A) The effectiveness of RNF6 plasmid transfection was evaluated via Western blot analysis in HeLa and SiHa cells. (B) Cell viability was evaluated using CCK-8 experiments. (C-E) The impact of RNF6 on colony formation, invasion, and migration abilities of CC cells was examined through colony formation experiments, transwell invasion assays, and wound healing assays in cells transfected with Flag-RNF6 or vector. *p < 0.05, **p < 0.01