Fig. 6

Overexpression of TBC1D31 induces tumor growth in nude mice in the presence of ZSCAN16 knockdown. A: The subcutaneous tumor volume was measured every four days during the subcutaneous tumorigenic assay in nude mice. B: After the tumorigenic assay and the nude mice were euthanized, the subcutaneous tumors were collected and weighed. C: IHC detected the expression of Ki67, N-cadherin, Snail, and Cleaved Caspase-3 in the tumor tissues of nude mice. D: TUNEL detected the apoptosis of the subcutaneous tumor cells in nude mice. E: IHC analysis of protein expression of ZSCAN16 and TBC1D31 in tumor tissues. All animal experiments were independently repeated five times. Comparisons between groups were analyzed by unpaired t-test (BCDE), and comparisons among multiple groups were analyzed by ANOVA (A), * p < 0.05 compared with the sh-ZSCAN16 + oe-NC group