Fig. 3

ZSCAN16 knockdown inhibits HCC progression in vivo. A: mRNA expression of ZSCAN16 in tumor tissues formed by Hep3B cells with sh-ZSCAN16 was detected by RT-qPCR. B: Subcutaneous tumor volume was measured every four days during the subcutaneous tumorigenicity assay in nude mice. C: After the tumorigenicity assay and nude mice were euthanized, subcutaneous tumors were collected and weighed. D: Expression of Ki67, N-cadherin, Snail, and Cleaved Caspase-3 in mouse tumor tissues was detected by IHC. E: TUNEL to detect apoptosis of subcutaneous tumor tissues in mice. All animal experiments were independently repeated five times. Comparisons between two groups were analyzed by unpaired t-test (A, C, D, E), and comparisons between multiple groups were analyzed by ANOVA (B). * p < 0.05 compared with the sh-Scramble group