Fig. 2

ZSCAN16 knockdown represses malignant biological behaviors in HCC cells. A: The infection effect of sh-ZSCAN16 in HCC cells was examined using RT-qPCR. B: The proliferation of HCC cell lines treated with knockdown ZSCAN16 was examined using a colony formation assay. C: Migration assay of HCC cell lines with knockdown ZSCAN16. D: Analysis of the invasive ability of HCC cell lines with knockdown ZSCAN16. E: Apoptosis in HCC cell lines with knockdown ZSCAN16 treatment was examined using TUNEL assay. F: Apoptosis in HCC cell lines with knockdown ZSCAN16 treatment was examined using flow cytometry. G: The protein expression of N-cadherin, Snail, and Cleaved Caspase-3 in HCC cell lines with knockdown ZSCAN16 treatment was examined using WB. All cell experiments were independently repeated three times. Comparisons between two groups were performed using the unpaired t-test (ABCDEF), and comparisons between multiple groups were analyzed by ANOVA (G). * p < 0.05 compared with the sh-Scramble group