Fig. 1

ZSCAN16 was significantly upregulated in human HCC cells and tissues. A: The volcano maps of DEGs in the GSE57957 and GSE46408 datasets. B: Intersection genes in human TFs and DEGs (p. adj. < 0.01) in GSE57957 and GSE46408 datasets obtained from Jvenn. C: Expression of ZSCAN16 in HCC was analyzed on UALCAN. D: RFS of HCC patients with high or low expression of ZSCAN16 was analyzed on Kaplan-Meier Plotter. E: mRNA expression of ZSCAN16 in human HCC tissues and paracancerous tissues was detected by RT-qPCR (n = 12). F: Comparison of ZSCAN16 protein expression in human HCC and paracancerous tissues by IHC scoring (n = 12). G, H: RT-qPCR and WB for detection of transcriptional and translational levels of ZSCAN16 in THLE-2 and HCC cell lines. All cell experiments were repeated three times independently. Comparisons between two groups were analyzed by paired t-test (EF), and comparisons between multiple groups were analyzed by ANOVA (GH). * p < 0.05 compared with adjacent tissue or THLE-2 cells