Fig. 4

c-MYC is an essential factor for CLK3-mediated tumor proliferation. A. IBs to detect the expression level of related proteins in Vector + shnc, CLK3 + shnc, CLK3 + shc-MYC#1, or CLK3 + shc-MYC#2 cells. B. Measuring the ATP expression level in four kinds of colorectal cancer cells. C. CCK8 assays to assess the proliferation ability of four kinds of colorectal cancer cells. D. RT-PCR to detect the mRNA level of identified genes. E. IBs to detect the expression level of related proteins in shnc + Vector, sgCLK3#1 + Vector, or sgCLK3#1 + c-MYC cells. F. Measuring the ATP expression level in three kinds of colorectal cancer cells. G. CCK8 assays to assess the proliferation ability of three kinds of colorectal cancer cells. H. RT-PCR to detect the mRNA level of identified genes. Statistical analysis was conducted using Student’s t-test. All IBs were performed three times, yielding similar results