Fig. 3

CLK3 positively activated MYC signaling. A. GSEA analysis to reveal the relationship between CLK3 and c-MYC signaling using TCGA and GEO database. B. Detecting the expression of identified proteins in Vector or CLK3 colorectal cancer cells. C. Detecting the expression of identified proteins in shnc or sgCLK3 colorectal cancer cells. D. IP and IB assays to confirm the binding between c-MYC and CLK3. E, F. RT-PCR to detect the mRNA level of identified genes. G. Detecting the expression of Wnt/beta-catenin pathway-related proteins in shnc or sgCLK3 colorectal cancer cells. H. Isolating nuclear component in Vector or CLK3 colorectal cancer cells, and IB to detect the expression of identified proteins. Statistical analysis was conducted using Student’s t-test. All IBs were performed three times, yielding similar results