Fig. 5

ZFP69B transcriptionally activates TLX1 expression in HCC cells. (A) The intersection of TFs with binding sites in the TLX1 promoter and enhancer regions downloaded on GeneCards and differentially expressed genes in the GSE87410 and GSE105130 datasets. (B) The heatmaps of intersecting TF expression in the GSE87410 and GSE105130 datasets. (C) The ZFP69B binding peaks on the TLX1 promoter were downloaded from the UCSC database. (D) Expression of ZFP69B mRNA in HCC tissues and their adjacent tissues was examined using RT-qPCR (n = 31). (E) positive staining of ZFP69B in HCC tissues and their adjacent tissues was analyzed using immunohistochemistry (n = 31). (F) ZFP69B mRNA in THLE-2, Huh-7, and MHCC97H cells was examined using RT-qPCR. (G) Enrichment of the TLX1 promoter in HCC cells with anti-ZFP69B antibody or anti-IgG control was analyzed using ChIP. (H) The binding relation between ZFP69B and TLX1 was examined using a luciferase reporter assay. (I) TLX1 mRNA in Huh-7 and MHCC97H cells infected with sh-ZFP69B was examined using RT-qPCR. Data represent the mean ± SEM of at least three independent experiments. **p < 0.01, ***p < 0.001, ****p < 0.0001. Differences were tested using a t-test (D, E, G, H, I) and the one-way ANOVA (F)