Fig. 3

NEAT1 reverses the anti-tumor effects of sh-BHLHE40 in CRC cells. A RT-qPCR detection of NEAT1 overexpression efficiency. B MTT assay for LoVo and HCT-15 cell viability. C Transwell assay for LoVo and HCT-15 cell migration ability. D Transwell assay for LoVo and HCT-15 cell invasion ability. E Western blot for E-cadherin, N-cadherin, and Vimentin expression in LoVo and HCT-15 cells. F Flow cytometry for LoVo and HCT-15 cell apoptosis. Results were expressed as magnitude of relative expression (means ± SD) from three independent experiments. A–F two-way ANOVA. *p < 0.05