Fig. 4

NUAK2 depletion triggers ferroptosis in A549 cells. (A) Lipid ROS was measured by BODIPY (581/591) C11 staining. (B) Lipid peroxidation was determined by TBARS method. (C) Fe2+ level in A549 cells was examined using an Iron Assay kit. (D) Ferroptosis-related proteins were detected using western blot. **p < 0.01, ***p < 0.001 vs. sh-NC