Fig. 5

NPRL2 depletion may induce mitotic catastrophe. A Left, representative images of the mitotic spindles in cells with or without NPRL2 depletion. Seventy-two hours after transduction, Ras-AI-T2 cells were fixed and immunostained with anti-γ-tubulin (green) and anti-α-tubulin (red). Nuclei were counterstained with DAPI (blue). Scale bar, 10 μm. Right, the percentages of cells displaying abnormal mitotic spindles. Results are the mean ± SD from at least 200 cells for each condition, as determined from three experiments. **p < 0.01 by Student’s t-test. B NPRL2 depletion increases the percentage of cells with multiple nuclei or micronuclei. Ras-AI-T2 cells that were transduced with control shRNA virions (pLKO.1) or depleted of NPRL2 (shNPRL2) for 96 h were used for detection and quantification of multiple nuclei (left) or micronuclei (right). The percentages (mean ± SD) of cells displaying multiple nuclei or micronuclei were determined using at least 500 cells from three independent experiments. *p < 0.05 and **p < 0.01 by Student’s t-test