Fig. 5

EV-delivered hsa_circ_0090081 enhances GC tumorigenesis. A TEM was used to observe the EVs isolated from AGS cells. B NTA analyzed the size distribution of EVs from AGS cells. C Western blotting analysis of key protein levels of EVs (CD9 and CD63) in EVs, AGS cell extracts, and supernatants. D The hsa_circ_0090081 levels in AGS cells treated with EVs or PBS were revealed via qRT-PCR assay. Cell viability, proliferation, migration, and invasion of AGS cells treated with EVs or PBS were revealed using CCK-8 assay (E), EdU assay (F), Transwell assay (G), and Transwell assay (H), respectively. *P < 0.05, **P < 0.001 vs. PBS