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Fig. 3 | Cell Division

Fig. 3

From: LncRNA NUTM2A-AS1 silencing inhibits glioma via miR-376a-3p/YAP1 axis

Fig. 3

LncRNA NUTM2A-AS1 negatively regulates miR-376a-3p in U251 cell line. A–C qRT-PCR was performed to analyze the expression of lncRNA NUTM2A-AS1 and miR-376a-3p in U251 cells. D MTT assay was conducted to assess the cell viability of U251 cells. E, F Flow cytometry was used to quantify the apoptosis of U251 cells. G Western blot assay was conducted to analyze the protein expression of Bax and Bcl-2. H qRT-PCR was conducted to analyze the mRNA expression of Bax. I qRT-PCR was conducted to analyze the mRNA expression of Bcl-2. **p < 0.01 vs. control-siRNA; ##p < 0.01 vs. inhibitor control; &&p < 0.01 vs. NUTM2A-AS1-siRNA + inhibitor control

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