Fig. 4
From: SYVN1 modulates papillary thyroid carcinoma progression by destabilizing HMGB1
SYVN1 regulates HMGB1 protein degradation via ubiquitination. (A) Prediction of potential E3 ligase of HMGB1. (B) Co-IP analyzed the protein interaction between SYVN1 and HMGB1 proteins in NPA87 cells. (C) Effect of SYVN1 on HMGB1ubiquitylation in NPA87 cells. (D) WB showed the expression of HMGB1 in PTC cells. (E) WB shows the interference effect of the pcDNA3.1-SYVN1 interference on the HMGB1 protein in NPA87 and TPC-1 cells. (F) The half-life of HMGB1 in different groups