Fig. 3

Klotho regulated oxidative stress in PE models. A The MDA levels and B GSH/GSSG ratio in PE patients’ placental tissues were determined by their corresponding detection kits. C The expression levels of anti-oxidant proteins (Nrf2, SOD2 and NQO1) in PE patients’ placental tissues were detected by performing Western Blot analysis. D The MDA levels and E GSH/GSSG ratio in hypoxic human trophoblasts were respectively determined by their kits. F, G The human trophoblasts were subjected to hypoxia, and Western Blot analysis was performed to examine the expression status of nuclear Nrf2, total Nrf2, SOD2 and NQO1 in the cells. Each experiment was repeated at least for three times, and *P < 0.05 was considered as statistical significance. Each experiment was repeated at least for three times