Fig. 1

Klotho was correlated with the development of PE in clinic and in vitro. A The contents of Klotho in PE patients’ serum were determined by ELISA analysis. The mRNA and protein levels of Klotho in PE patients’ placental tissues were respectively determined by performing B Real-Time qPCR and C Western Blot analysis. The human trophoblasts were subjected to hypoxia treatment, and D, E Real-Time qPCR and F, G Western Blot was used to detect the expression levels of Klotho protein in those cells in vitro. Each experiment was repeated at least for three times, and *P < 0.05 was considered as statistical significance. Each experiment was repeated at least for three times