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Fig. 3 | Cell Division

Fig. 3

From: Polyploid cell dynamics and death before and after PEG-treatment of a NIH/3T3 derived culture: vinblastine effects on the regulation of cell subpopulations heterogeneity

Fig. 3

Cell heterogeneity and adhesion variability. Phase contrast micrographs (a–d) and fluorescence microscopy (e, f) of PEG-treated NIHs cells. In a, the morphological aspect of the culture before trypsinization; in b and c, the effects of trypsin action for 7 min are shown: after this incubation time, cells with large dimensions are still adherent, while the small cells are in suspension. In c, an area of the growth substrate showing the persistence of large cells after trypsinization for 7 min is shown. In d, it is possible to observe the detachment of large cells after 15 min of trypsinization. In e and f, immunofluorescence techniques allow us to evaluate cytoskeleton organization in giant cells: e, microtubules; f, actin microfilaments. Scale bar in a: 40 µm; in b and d: 10 µm; in c, e, and f: 20 µm

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Cell Division

ISSN: 1747-1028

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