Fig. 5

SGO1 modulates epithelial-to-mesenchymal protein expression. Western blot showing characterization of shRNA SGO1 in MDA-MB-231 cells (a). The graph shows the densitometry of the Western blot analysis (b). Western blot showing the expression of epithelial markers E-cadherin (E-cad) and ZO1, and mesenchymal marker Vimentin (Vim) in MDA-MB-231 cells treated with shRNA Control or shRNA SGO1 (c). The graph shows the densitometry of the Western blot analysis (d). Western blot showing the expression of EMT transcription factors Snail, Slug, Zeb1, and Twist1 in MDA-MB-231 cells treated with shRNA Control or shRNA SGO1 (e). The graph shows the densitometry of the Western blot analysis (f). For all graphs, error bars represent mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 were considered statistically significant. N.S. stands for not significant. A Student t-test (unpaired, two-tailed) was used to compare differences between groups