Fig. 2

Knockdown of RalA/B affects mouse oocyte maturation. A Real-time quantitative PCR result of RalA and RalB mRNAs expression level in RalA/B-KD group and control group. The relative mRNAs expression of RalA and RalB were significantly decreased in RalA/B-KD group. *P < 0.05.**P < 0.01, ***P < 0.001. B Western blot result of the protein expression of RalA/B after RalA/B siRNAs injection. The band intensity analysis for the RalA/B after RalA/B siRNAs injection. Compared with the control group, the protein expression of RalA/B was significantly decreased in RalA/B-KD group. *P < 0.05. C The typical picture for the oocytes polar body extrusion after RalA/B siRNAs injection. Bar = 80 µm. D The rate of polar body extrusion after RalA/B siRNAs injection. The rate of polar body extrusion was significantly reduced after RalA/B siRNAs injection. *P < 0.05