Fig. 1

a Equal amounts of TCA extracts obtained from cells treated in the presence or the absence of HU (150 mM) or MMS (0.03%) for 90 min were resolved on either regular (Rad53) or Phos-Tag (Exo1-Myc) 8% SDS-polyacrylamide gels. Protein expression was examined by immunoblot (IB) with appropriate antibodies. b Equal amounts of TCA extracts obtained from the indicated strains treated in the presence or the absence of HU (150 mM) were resolved as in (a). Exo1 was visualized with an antibody to the Myc-tag