Figure 1

IMAGe-mutant CDKN1C stabilizes CDKN1C protein. Cells were transiently transfected with plasmids expressing CDKN1C wild-type or IMAGE-mutants (p.K278E and p.F276V). After addition of cycloheximide (CHX) at 100 μg/mL to the culture medium, cells were harvested at various times and processed by Western blot analysis of CDKN1C protein.