Figure 5

The protein content in HCT116 and WI-38 cells treated with TSA. Representative Western blot of HP1α, HP1β, H3, H3K9me3, H3K9ac and CENP-A levels after treatment with 1 μM TSA for 24 or 48 h. The experiments were conducted in 3 independent determinations that were performed in duplicate for each experimental condition; the asterisk indicates p < 0.05 compared with the value of the control (CTR), as obtained by Student’s t-test.