Figure 3
From: Cohesin loading factor Nipbl localizes to chromosome axes during mammalian meiotic prophase
Nipbl translocates during pachytene stage in spermatocytes. Immunofluorescence images of chromosome spreads stained with antibodies against Nipbl (green) and other marker proteins. More than 5 nuclei were examined in each stage. DNA was counterstained with DAPI. E, early; M, mid; L, late. Scale bars 10 μm. (A) Anti-γH2AX (red) marks double strand breaks (DSBs). Note that anti-γH2AX highly accumulates at the sex body during the stages shown. (B) Rad51 (red) marks recombination intermediates. (C) Mlh1 (red) marks late recombination nodules.