Figure 4

Mitotic cyclinA is not degraded in lmg mutants. Images show cyclin A (red in A, B, C, monochromatic in D, E, F), and tubulin (green in A, B, C, monochromatic in G, H, I) localizations as well as DNA staining (blue in A, B, C, monochromatic in J, K, L) in mitotic cells. In wild-type cells (n = 29) the cyclin A staining is visible in prophase or prometaphase (A and D), but undetectable in metaphase cells (B and E). In metaphase-like lmgJ023 cells (n = 17) cyclin A staining is intense (C and F).