Figure 7
From: The WW-HECT protein Smurf2 interacts with the Docking Protein NEDD9/HEF1 for Aurora A activation
Depletion of Smurf2 results in the lack of Aurora A activation and delayed mitotic entry. HeLa cells were transfected with siRNA against Smurf2 or control dsRNAs (siNS), synchronized at early S phase by a double thymidine protocol, and released into synchronized progression toward mitosis. Cells were harvested at the indicated times after release for immunoblotting with the antibodies shown on right.