Figure 6
From: The WW-HECT protein Smurf2 interacts with the Docking Protein NEDD9/HEF1 for Aurora A activation
Depletion of Smurf2 decreases the activated pool of Aurora A. Lysates of control and Smurf2 depleted HeLa cells were first immunoprecipitated with pan-Aurora A antibody and then western blots were probed with antibody specific for Thr288 phosphorylated active Aurora-A.